Nutritional Consequences of Critical Illness Myopathies Apoptosis of Skeletal Muscle on Steroid-Induced Myopathy in Rats

نویسندگان

  • Min-Cheol Lee
  • Jae-Hyoo Kim
چکیده

Apoptotic cell death of differentiated skeletal muscle has been reported in an experimental steroidinduced myopathy of rats. To investigate the underlying molecular changes in the apoptosis of skeletal muscle, in situ end labeling (ISEL), Fas expression, and Western blot analysis for apoptosis-related proteins in the soleus muscle of triamcinolone acetonide (TA)-induced myopathy of rats were studied. Proteins for Western blot analysis included Fas-associated death domain (FADD) and caspase 8 for extrinsic pathway, as well as Bcl-2, Bcl-XL, Bax, Bad, Bid, Akt, p-Akt, and caspase 9 for intrinsic pathway. ISEL-positive myonuclei in TA-treated rats were 1.8 1.2%, whereas Fas-positive muscle fibers were 4.5 2.0%. One-fourth of Fas-positive muscle fibers had ISEL-positive myonuclei. Levels of FADD, Bax, Bad, and Bid were substantially increased in the TA-induced myopathy group, whereas Bcl-2, Bcl-XL, Akt, p-Akt, and caspase 9 did not change between control and myopathy groups. Caspase 8 activity increased in the myopathy group. These findings indicate that apoptosis of skeletal muscle in TA-induced myopathy may be triggered by Fas-Fas ligand signals and promoted mainly by overexpression of the pro-apoptotic molecules of FADD and caspase 8 involving the extrinsic pathway. The apoptotic process presented in this study supports a direct, nongenomic effect of a glucocorticoid on cellular membranes leading to cellular apoptosis rather than genomic effector mechanism of steroid hormone mediated by cytosolic steroid receptors. J. Nutr. 135: 1806S–1808S, 2005.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Histochemical And Electron Microscopic Diagnosis Of Mitochondrial Myopathy: The First Case Report From Iran

  Muscle tissue, skeletal muscle as well as cardiac muscle, is commonly affected in mitochondrial disorders. One explanation for this observation is that muscle tissue has a high-energy demand and therefore is more sensitive to a deficiency of mitochondrial energy production than some other tissues. In mitochondrial disorders, skeletal muscle tissue may be affected primarily by defective respi...

متن کامل

Myopathies in critical illness: characterization and nutritional aspects.

Myopathies related to critical illness have received increasing recognition over the past decade and are common in patients even after a brief period in the intensive care unit. Recent studies have revealed that myopathies in the critically ill may in fact be more prevalent than neuropathies and that morbidity and mortality may be greater. Protein catabolism, an increase in urinary nitrogen los...

متن کامل

Apoptosis of skeletal muscle on steroid-induced myopathy in rats.

Apoptotic cell death of differentiated skeletal muscle has been reported in an experimental steroid-induced myopathy of rats. To investigate the underlying molecular changes in the apoptosis of skeletal muscle, in situ end labeling (ISEL), Fas expression, and Western blot analysis for apoptosis-related proteins in the soleus muscle of triamcinolone acetonide (TA)-induced myopathy of rats were s...

متن کامل

Simultaneous Effect of Resistance Training and Stem Cell Injection on Blood Glucose Levels, Insulin Resistance, Caspase 3 And 7 As Indicators of Skeletal Muscle Apoptosis in STZ-Induced Male Diabetic Rats

Background: The aim of the present study was to investigate the simultaneous effect of resistance training and stem cell injection on the levels of some indicators of skeletal muscle apoptosis in STZ-induced diabetic male rats. Methods: In this study, 30 rats were randomly divided into 5 groups. Rats in the diabetic group and the diabetic group + stem cell injection had a total of 17 sessions...

متن کامل

Effect of resistance training on structural injuries of skeletal muscle induced by different doses of Boldenone in male rats

Background and Aims: The use of anabolic steroids is common among athletes who perform resistance training; nonetheless, the effect of their abuse, along with resistance training, on the structural destruction of skeletal muscles is not fully understood. The present study aimed to assess the effect of different doses of Boldenone, along with resistance training, on muscle injury. Materials and...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2005